Jarrell Miller has failed a second drugs test, which looks set to end his hopes of facing Anthony Joshua at Madison Square Garden on June 1.
Miller was due to fight IBF, WBA, and WBO champion Joshua before an "adverse finding" from a sample taken on March 20, threw his participation in the bout into doubt.
After being denied a licence to fight by the New York State Athletic Commission, the American boxer said he had never knowingly taken a banned substance, adding that he intended to appeal against the decision to revoke his licence.
Joshua's promoter, Eddie Hearn, has now revealed that he has learnt of a second alleged doping violation by Miller, meaning that fight is now all but dead in the water.
"We have now received news from VADA that Jarrell Miller has now failed a second separate test for a further substance. AJ's June 1 opponent will be announced next week - if you don't think you can beat him clean then delete my number!" Hearn wrote on Twitter.
A statement released to Sky Sports News later on Friday evening from Jarrell Miller's promoters, Salita Promotions, read: "Salita Promotions and Greg Cohen Promotions are very disappointed in these findings and feel awful that fight fans will be deprived of what would have been a great fight.
"It's a terrible outcome and unfortunate situation for all parties involved."
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